/Blog/ACS NANO reports on a new method for accurately assessing cancer invasion and metastasis
keyword：ACS NANO reports on a new method for accurately assessing cancer invasion and metastasis
Recently, ACS NANO (IF 13.7) published an article titled "The Precise Diagnosis ofCancer Invasion/ Metastasis via 2D Laser Ablation Mass Mapping ofMetalloproteinase in Primary Cancer Tissue", which discover an innovative diagnostic method for cancer invasion andmetastasis.
In this research, scientists developedpeptide-coated Au clusters with intrinsic red fluorescence and a specific masssignal. When these clusters labeled MT1-MMP in tumor tissue sections derivedfrom the xenograft lung carcinoma model, human lung carcinoma and human renalcarcinoma, they could directly observe MT1-MMP via optical fluorescencemicroscopy and quantitatively detect the MT1-MMP expression level via laserablation inductively coupled plasma mass spectrometry 2D mapping (2D-LA-MassMapping). By observing and quantifying the MT1-MMP expression level in primaryhuman lung carcinoma and human renal carcinoma tissue sections, they preciselyassessed the risk of primary tumor invasion/metastasis. The experimentalprocedure is as follows:
They demonstrated that the expression level of MT1- MMP inprimary tumor tissue is well correlated with the tumor regional invasion/remotemetastasis both for lung adenocarci-noma and renal cancer. Therefore, earlydiagnosis of primary tumor invasion/metastasis can be achieved by 2D-LA-Mass Mapping of MT1-MMPin tumor tissues. This method will have major benefits for clinical therapy andprognosis of primary tumor patients. They developed a method to construct Auclusters using CL peptides. Their mass mapping method of MT1-MMP in primarytumors had excellent accuracy compared with traditional H&E/IHC/IFassessment.
They designed a functional peptide to construct Au clusterprobes. The peptide sequence is H2N-CCYHWKHLHNTKTFL-COOH (CL). The CC captures Auclusters, and the HWKHLHNT- KTFL can specifically target MT1-MMP. The CL-coatedAu (Au-CL) clusters have the following functions: (1) specific targeting ofMT1-MMP by the CL peptide; (2) in situ fluorescent labeling of MT1-MMP proteinon cells/tissues as Au clusters with red light emission; and (3) precisequantification of MT1-MMP membrane protein on cells/tissues as Au clusters withspecific mass signals. Furthermore, a laser ablation inductively coupled plasmamass spectrometry 2D mapping (2D-LA-Mass Mapping) instrument was used toquantify the MT1-MMP level in primary tumor tissue sections.
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Article link address：https://pubs.acs.org/doi/10.1021/acsnano.8b05584
The peptide (H2N-CCYHWKHLHNTKTFL-COOH) was synthesized by asolid-phase and purchased from ONTORES BIOTECH.
Founded in 2009, ONTORES BIOTECHNOLOGIES is a world leadingbiotechnology company. We are committed to providing professional high qualitypeptide products and peptide customized services for global customers. TheONTORES platform provides integrated solutions for the development ofpolypeptide drug technologies, from technology development to small and pilottrials of clinical pharmaceutical products and commercial scale drug productionneeds, helping our partners realize the unlimited possibility of exploring newdrugs.
