What is net peptide content?
It is important to understand the difference between net peptide content and total peptide content.The net peptide content is the percentage of peptides relative to nonpeptidic materials, mostly counterions and moisture. The net peptide content can be determined by amino acid analysis. Please place a request for a quote if you require this service. Usually, hydrophilic peptides absorb tiny amounts of moisture even under strict lyophilization conditions. Net peptide content may vary from batch to batch depending on the purification and lyophilization processes.
What if some problem happens during the synthesis of my specific peptide?
Each peptide has its specific characteristics and not all outcomes can be anticipated. If something goes wrong during the synthesis and we cannot deliver your peptide on time, we will inform you as soon as possible.
How may I place my order?
Ontores needs the sequence, desired purity, desired quantity, and preferred payment information. If you have a PO (Purchase Order) number or credit card, you can send us the sequence by email and fax us the PO. If you like, you may view Ordering Support.
How are peptides synthesized?
Unlike the natural protein synthesis, peptides are synthesized from the C to N terminus. At LifeTein?, peptide synthesis is performed using PeptideSyn technology based on Fmoc or t-Boc chemistry to protect the alpha amino group. The deprotection agent (piperidine for Fmoc, TFA for Boc) frees the alpha amino group in preparation for coupling the next amino acid in the sequence. This reveals a new N-terminal amine to which the next amino acid may be activated by one of several reagents, forming a peptide bond. When the synthesis is complete, peptides are cleaved from the resin and de-protected. Peptides are then precipitated, washed, and lyophilized.
What is peptide purity?
Peptide purity is the amount of the target peptide as determined by HPLC at 214 nm, where the peptide bond absorbs. Water and residual salts are not detected by UV spectrophotometer. Other impurities that can be found in the content include deletion sequences (shorter peptides lacking one or more amino acids of the target sequence), truncated sequences (generated by capping steps to avoid the formation of deletion peptides), and incompletely deprotected sequences (generated during the synthesis or the final cleavage process).